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Seems like the fact that 13C methyl selection of acetate was directly transformed into 13CH4 (13CH3COO+H+13CH4+CO2). These observations revealed that the dominant methanogenic pathway in the control microcosms was syntrophic acetate oxidation in addition to hydrogenotrophic methanogenesis, while acetoclastic methanogenesis was the dominant methanogenic pathway inside CO2 injected microcosms. The of carbon isotopic fractionation between CH4 and DIC within the unlabeled control (C=83.31.3) and CO2 injected (C=10.91.0) microcosms during incubation (Supplementary Table S2) were also in conjuction with the hydrogenotrophic and acetoclastic methanogenesis, respectively16.

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